DearAhmed. It has already finished successfully. I see some differences
between samples, which sounds great.
However, I am not sure of one question. In the column “positives” that I
interpret as the number of times this specific sequence has been found, I
always find a number of 32 (or blank in many samples and many isoforms) or a
number around 1000 in others. So it seems to me that the program is imputing a
value instead of counting . Am I right? So, the results will only say if the
isoform has been found positively or not, without quantitation?
Thanks for your help